Water extraction studies were carried out on Mucuna beans (Mucuna pruriens) to determine the extraction rate of L-dopa as a function of bean. Extraction of bioactive principle from Mucuna pruriens seeds The L-DOPA could be obtained in good yield on extraction with EtOH-H2O. Abstract: Mucuna pruriens seeds are noted to be a natural source of L-DOPA and are also used as a substitute for the synthetic L-DOPA. In the present study;.
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L-DOPA extracted from seeds of Mucuna is a constituent of more than indigenous drug formulations and is more effective as drug than the synthetic counterpart. Quantification was done at nm using absorbance reflectance mode. The method was validated for prruriens, precision and repeatability.
Extraction of bioactive principles from Mucuna pruriens seeds.
Mean recovery was The method was used to study variation in fifteen accessions of Mucuna germplasm collected from different geographical regions. Fabaceaecommonly known as Kewanch or Kaunch, Atmagupta and Velvet bean, is a climbing annual legume, endemic in India and in other parts of the tropics including Central and South America. About fifteen species of Mucuna are found in the forests and plains of India.
It is useful as a green manure and cover crop and is also grown for its pods and young leaves, which are used as vegetable and fodder[ 1 ]. All the parts of Mucuna contain valuable medicinal properties[ 23 ] and there is heavy demand of Mucuna in Indian drug market. In Ayurvedic system, the kaunch seeds and roots are used for the treatment of diseases of central nervous system and also as an anthelmintic[ 45 ].
Its different preparations from the seeds are also used for the management of ageing, rheumatoid arthritis diabetes, male infertility and nervous disorders. In addition seeds contain tryptamine, 5-hydroxytryptamine 5-HTmucunine, mucunadine, prurienine and prurieninine[ 67 ].
Though there are reports of several methods for the determination of L-DOPA in biological fluids and pharmaceutical preparations, such as by spectrophotometer[ 8 ], HPLC[ 910 ] etc.
Nevertheless, HPLC and other mentioned techniques have often suffered from diverse disadvantages of high cost of analysis, selectivity, with complex sample preparation procedures, and long analysis time.
In the recent past high performance thin layer chromatography HPTLC has emerged as an important tool for the qualitative and quantitative phytochemical analysis of herbal drugs for quality control.
It is carried out within a short period of time, requires small quantity of solvents, and allows for the analysis of a large number of samples simultaneously. The ability of HPTLC to analyze many samples in parallel has the advantage over other techniques because separation of 10 to 20 samples takes the same time as the separation of one sample.
It can detect as low as upto nanograms compound. Therefore, the aim of the present study was to develop and validate a simple, rapid, sensitive, precise and economical HPTLC method for the quantitative determination of L-DOPA in seed extracts of Mucuna pruriens.
Standard working solution was prepared by diluting stock solution with 0. Fifteen accessions of Mucuna germplasm representing three species namely, M. These were collections from different geographical regions of India and three exotic accessions were from Australia, USA and Italy. The seed coat colour varied from white, cream, black, and brown among seeds of these collections. Seeds of Mucuna pruriens were dried and powdered after removing seed coats. One gram of the powdered seeds was refluxed with 50 ml 0.
The extract obtained was filtered using whatman filter paper and the residue was again refluxed with fresh 50 ml of 0. The extract was filtered and both the filtrates were pooled to make total volume of the extract ml with 0. Experimental conditions were optimized. The linear ascending development was carried out in a twin trough chamber which was presaturated with 12 ml mobile phase of n-butanol: The plates were developed upto 8 cm and then air dried.
In order to determine the detection limit LOD and the quantification limit LOQanalyte concentrations corresponding to the lower part of the linear range of the calibration curve were used. The method was validated according to ICH guidelines for precision, accuracy and repeatability[ 1314 ]. Different compositions of the mobile phase were tested and the good resolution of L-DOPA was achieved by using mobile phase of n-butanol-acetic-acid-water 4: The peak purity of L-DOPA was assessed by comparing the spectra of standard and sample track at three different levels, i.
Results of regression analysis on calibration curve and detection limits are presented in Table 2. The accuracy of the method was ascertained by carrying out recovery studies by standard addition method.
A known but varying amount of standards from L-DOPA was added to the preanalyzed sample and analyzed according to the proposed method. The recoveries were found between Similarly, the inter-day precision was tested on the same concentration levels on two days and the RSD values were 1. L-DOPA content was found between 2.
This method allows a large number of samples to be measured simultaneously with a very good accuracy, sensitivity, and precision. It is very important especially in quality control. This method can also be used for the extractio determination of L-DOPA in herbal extract and its formulations. National Center for Biotechnology InformationU. Indian J Pharm Sci. Author information Article notes Copyright and License information Disclaimer. This is an open-access article distributed extractio the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.
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Abstract Mucuna pruriens Linn. Open in a separate window.
Footnotes Raina and Khatri: The Wealth of India. The Wealth of India; p. Indigenous Drugs of India. Bhagwati Pocket Books; Compendium of Indian Medicinal Plants; p.
Sathiyanarayanan L, Arulmozhi S. Misra L, Wagner H. Alkaloidal constituents present in Mucuna pruriens seeds. Extractions of bioactive principles from Mucuna pruriens seeds.
Extraction of bioactive principles from Mucuna pruriens seeds.
Indian J Biochem Biophys. Siddhuraju P, Becker K. Rapid reversed-phase performance liquid chromatographic method for the quantification of L-DOPA, non—methylated and methylated tetrahydroisoquinoline compounds from Mucuna beans.
International Conference of Harmonization, Geneva. Quality control of herbal drugs; pp. Prakash D, Tewari SK. J Med Aro Plant Sci.
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